Murine pancreatic islet isolation.

Name Type Company Catalog Number Comments Dithizone reagent Dissolve 5mg Dithizone in 5 ml of DMSO in a 50 ml conical tube. Add 45 ml of normal saline and mix gently. Filter with a 60 cc syringe and a 0.22 "m syringe filter into a new 50 ml tube. Store at 4 OC, until use. Add 1 ml to each 35 mm counting Grid-plate, when needed. PBS Buffer HBSS Buffer Isolation soln. Buffer HBSS containing 10 mM of HEPES, 1 mM of MgCl2, 5 mM of Glucose, pH 7.4 Wash soln. Buffer Isolation buffer containing 1mM CaCl2 Collagenase P Boehringer Ingeheim 1213873 Dissection tools Hemostat clamp (n=2), Forceps (n=2), Scissors (for skin incision an for taking out pancreas from adjacent tissue) Syringe sterile, 5ml, 1/mouse. 30 G needles sterile, 1/syringe siliconized glass vials w/ Teflon cap Fisher Scientific 213-018-54 sterile, 1/mouse